Cardiogen 20mg

Description

Description & Mechanism

Cardiogen is a short synthetic tetrapeptide employed as a molecular probe for investigating tissue-specific transcriptional regulation in cardiac-derived cell and tissue systems. It is used experimentally to study modulation of gene-expression programmes in cardiomyocyte and cardiac-fibroblast models, including transcripts associated with contractile proteins, calcium handling and oxidative-stress defence.

Consistent with the Khavinson short-peptide model, Cardiogen is studied as an agent that may translocate intracellularly and engage DNA/chromatin to influence promoter activity. As a low-molecular-weight linear peptide it is used in experimental designs focused on transcriptional regulation and cellular stress signalling; observed effects depend on concentration, model system and exposure conditions.

Applications in Research

• In-vitro gene-expression studies in cardiomyocyte and cardiac-fibroblast cultures
• Cellular models of oxidative stress and cardiomyocyte apoptosis / protein synthesis
• Preclinical models of cardiac aging and ischaemia-related gene-expression changes
• Probe for short-peptide / chromatin interaction studies

Specifications Summary

Handling, Reconstitution & Stability

• Weigh under dry conditions; peptide is hygroscopic
• Reconstitute in sterile water or appropriate buffered aqueous solution depending on assay requirements
• Avoid vigorous agitation during dissolution
• Filter sterilize if required (e.g. 0.22 µm) prior to use
• Aliquot and store reconstituted solutions at –20 °C or below to minimise degradation
• Avoid repeated freeze–thaw cycles

Precautions & Notes

• Experimental behaviour is dependent on concentration, model system and exposure conditions
• Appropriate experimental controls are recommended
• Prolonged exposure to light, elevated temperature or aqueous environments may reduce stability
• Intended strictly for laboratory research use; not for human or veterinary application